Biotin Question

This topic has 4 replies, 2 voices, and was last updated 2 months, 3 weeks ago by Terri Warren.

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- January 1, 2023 at 7:53 am #79895
  
  June1234
  Participant
  
  Hi Terri! Pleasure to meet you virtually. My question is in regards to biotin interference. Western Blot still does NOT use biotin-streptavidin system, correct? I am taking 3000mcg of Biotin (Nutrafol) and tested negative for both 1&2 via WB. Could the Biotin have caused a false negative WB?
  
  Thank you kindly and happy new year!
- January 2, 2023 at 5:26 am #79937
  
  Terri Warren
  Keymaster
  
  Biotin does not impact the western blot, correct. I’ve checked
  
  Terri
- January 2, 2023 at 7:19 am #79940
  
  June1234
  Participant
  
  Thank you Terri!
- January 3, 2023 at 11:14 am #79950
  
  June1234
  Participant
  
  One more anxiety driven question, is the reason the WB is not affected because it does not use the biotin-streptavidin system? Whereas other tests do?
- January 9, 2023 at 8:47 am #79998
  
  Terri Warren
  Keymaster
  
  Correct. Western blot is an entirely different process.
  
  Terri
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