I am stunned that someone did an antibody test on a 3 year old. We know that half of the positive in children are false positives though this is a high positive. Igg tests for herpes are NOT indicated for children. Biotin nor vitamin D will skew western blot results. And there is no evidence that vitamin D would impact testing. People who have high HSV 1 index values may well have have false positive HSV 2 test results. The vaccine should not impact herpes testing either.