› Forums › Herpes Questions › Testing confusion. Did I get a false positive or do I have Hsv2? › Reply To: Testing confusion. Did I get a false positive or do I have Hsv2?
If I were you, I would copy the information from the CDC about the immunoblot: here it is.
The most commonly used test,
HerpeSelect HSV-2 enzyme immunoassay (EIA), often is
falsely positive at low index values (1.1–3.0) (457–457). One
study reported an overall specificity of 57.4%, with a specificity
of 39.8% for index values of 1.1–2.9 (458). Because of the
poor specificity of commercially available type-specific EIAs,
particularly with low index values (<3.0), a confirmatory test
(Biokit or Western blot) with a second method should be
performed before test interpretation. Use of confirmatory
testing with the Biokit or the Western blot assays have been
reported to improve accuracy of HSV-2 serologic testing (459).
The HerpeSelect HSV-2 immunoblot should not be used for
confirmation because it uses the same antigen as the HSV-2
EIA.
If confirmatory tests are unavailable, patients should be
counseled about the limitations of available testing before
obtaining serologic tests, and health care providers should
be aware that false-positive results occur. Immunoglobulin
M (IgM) testing for HSV-1 or HSV-2 is not useful because
IgM tests are not type-specific and might be positive during
recurrent genital or oral episodes of herpes (460). Therefore,
HSV IgM testing is not recommended.
I would copy this, send it in, and ask for a refund. This is total BS that they are doing this.
With a negative supplemental and limited funds right now, I think you can forego the blot.
However, we only have about 25 cases of people who had negative supplementals who did western blots and came back negative (I don't believe we have any who came back positive). That's a small sample, but I sure hope it holds
Terri